China3D printingNet, June 29, scientists from the California Institute of Technology and the University of California, San Francisco have developed DNA-laden3D printingThe structure can guide chemotherapy drugs to the affected organs and away from healthy tissues.
The new device, nicknamed “chemical filter”, can be injected into patients during intra-arterial chemotherapy to expel the affected organs and prevent toxic substances from entering their bloodstream. Thanks to its genomic DNA coating, the team said that ChemoFilters can efficiently capture adriamycin and protect patients from off-target toxicity.
The team’s 3D printed SEM image of the drug absorbent.
By coating their devices with genomic DNA, the team was able to give them drug-absorbing properties. The picture comes from Applied Materials and Interface Magazine.
Chemotherapy filter innovation
According to a study by the American Cancer Society, by 2040, the number of cancer deaths will reach 16 million. This statistic raises doubts about the effectiveness of current treatment methods. Although chemotherapy has become a powerful tool to fight disease, its success is still limited by “systemic toxicity”. This phenomenon is that drugs that are not absorbed by cancer cells instead kill healthy cells, often leading to organ damage.
To make matters worse, many chemotherapy drugs show greater efficacy at higher doses, forcing clinicians to choose between maximizing tumor suppression and avoiding damage to surrounding tissues. Where more targeted treatments are developed, they are also accompanied by lengthy development times and other debilitating side effects, which may prevent patients from receiving treatment.
To solve this problem, scientists at the University of California, San Francisco created a chemical filter for the first time in 2014. It is wrapped in external DNA so that chemical drugs will attack them instead of healthy cells. However, despite the team’s initial design Iterative, but there are limitations in the use of drug-binding materials that hinder the production of upgraded equipment.
Recently, researchers at the nearby University of California, Berkeley discovered that sulfonated 3D printed copolymers have excellent drug capture potential. Drawing inspiration from the success of their compatriots, the teams at the California Institute of Technology and the University of California, San Francisco have now proposed coating polyacrylates with DNA to make them more effective in in vitro drug capture applications.
The researchers 3D printed the DNA loading and control components.
The team found that electrostatic interaction is the most effective way to achieve DNA binding. The picture comes from Applied Materials and Interface Magazine.
“Chemical filter” loaded with DNA
First, the scientists used Autodesk Ember DLP 3D printers and PR48 resin to produce a set of 12 mm (width) x 2.5 mm (height) cubic lattices. Each device has a 16 x 16 x 3 internal unit with an opening of approximately 500 μm, making it large enough to allow cells to flow through, and their overall structure is small enough for deployment outside the body.
When ready, the team used two different techniques to coat their parts with genomic DNA. In the first method, the untreated lattice is immersed in an acidic DNA solution, while in the second method, the researchers combine electrostatic interactions and UVC cross-linking to bind the negatively charged genome to their On the device.
Before scientists even started testing, they knew that the former caused DNA strands to aggregate into fiber clusters instead of covering the surface of the lattice, resulting in a reduction in the number of layers. In order to maximize the drug absorption properties of their devices and prevent potential DNA “leakage”, the team soaked them in PBS before testing, during which they exposed them to doxorubicin under in vitro conditions .
Compared with ordinary lattices, the researchers’ new devices proved to be able to capture almost twice the amount of anticancer drugs, leaching 100 pg of genomic DNA per square millimeter of material every 30 minutes, leading the team to conclude that they represent “a move toward These devices are transformed into clinical applications.”
The team concluded in their paper: “The use of equipment to reduce off-target toxicity in chemotherapy is of great significance and has the potential to improve the way we manage cancer. We hope that this concept of drug capture can be extended to address the medical needs Address other issues of off-target toxicity.”
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