Concentration gradient microfluidic chip based on 3D printing for rapid drug susceptibility detection of microorganisms

This research paper focuses on the rapid drug susceptibility detection of microorganisms. Antibiotic resistance is a serious challenge to global public health security.Accelerated evolution of drug resistance in pathogens increases
clinical
Medication difficulty and patient mortality in the treatment of multidrug-resistant infections. Obtaining the antibiotic drug sensitivity results of microorganisms in time is of great significance for the accurate diagnosis and drug treatment of clinical multidrug-resistant infections. In this study, a microdroplet chip based on flow resistance was designed, combined with the application of resazurin biological indicator, which can indicate the growth of microorganisms under different concentrations of antibiotics within 5 h. The chip has several independent retention chambers, which can automatically generate antibiotic concentration gradients and form independent droplets for detecting bacterial susceptibility. The chip simplifies control operations and device integration, shortens drug sensitivity detection time compared to traditional methods, and has good application prospects.

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