FLUICELL collaborates with Swedish researchers to 3D bioprint nerve cells into complex patterns

China3D printingNet January 8th, scientists from medical technology company Fluicell have cooperated with clinical research and development company Cellectricon and Karolinska Institute University in Sweden to combine nerve cellsbiology3D printingInto complex patterns.
Using the microfluidic print head on the Fluicell Biopixlar platform, researchers can accurately arrange rat brain cells in a 3D structure without compromising their viability. The resulting brain tissue can be used to simulate the progression of neurological diseases or test the efficacy of related drugs.

Cellectricon CEO Mattias Karlsson said: “We have been using Biopixlar to develop protocols for printing different types of neuronal cells, and we are very satisfied with its performance. “This exciting technology has the potential to be broadly central and In vitro modeling of PNS-related diseases opens up a whole new way. “


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Using Fluicell’s Biopixlar platform, scientists can accurately3D printingA series of cell-based structures (pictured). Picture from Fluicell.


Fluicell’s precision3D printingCraft

Fluicell was spun off from Chalmers University of Technology in 2012 and chose to launch its first bioprinter together with Biopixlar in November 2019. This machine is called the “all-in-one discovery platform” and can print multiple different units in one run while maintaining high accuracy and resolution.
Fluicell’s system includes a robotic arm, a motorized platform, and a gamepad interface, allowing users to manually place cells. The precision provided by the machine makes it a potential thin-page printing application, and since its launch, the company has been seeking to demonstrate its clinical capabilities.

The company signed a beta agreement with Cellectricon to evaluate the use of its technology in neurological research through the agreement. Considering that mapping brain diseases usually requires the use of multiple cell types, the partners believe that Biopixlar can be used to improve the prognosis of patients.
Previous methods of printing artificial tissues have focused on extrusion or laser-based methods, many of which require the use of sacrificial gels to limit their accuracy. Instead, using a microfluidic process, the Fluicell team found that they were able to precisely control the deposited cells without inhibiting their growth.



Biopixlar’s microfluidic nozzles (shown in the picture) enable the research team to recirculate the cells so that they can achieve high accuracy during the test. Picture from “Science Report” magazine.


Test the Biopixlar printer

The main advantage of Biopixlar is that it has a print head with three independent chambers, and the team was able to avoid cross-contamination during the test. These cells are also confined to the circulating flow, which means they can only deposit after they interact firmly with the surface.
Using this method, scientists are able to collect and reuse any unattached cells while maintaining a high degree of control over the cell pattern. In addition, through careful pressure balancing, the team found that they can adjust the scale and layout of the organization in real time, making the process extremely adaptable.

In a more advanced test, the researchers printed both adult skin and cancer cells into 2D tissue, with a survival rate of over 99%. Once exposed to retinoic acid (RA) skin drugs, the tumor-producing CK 10 protein in cancer cells will drop by 25%, which proves the potential of this tissue in clinical research.

By using polylysine (PLL) as a cellular “glue” to attach to several of these two-dimensional tissues, the team was finally able to layer them into living brain cancer models. According to scientists, their novel PLL-based method demonstrates that Biopixlar is sufficiently non-invasive and accurate to ensure its widespread use as a diagnostic tool.


Bioprinting soft tissue structure

Although full size3D printingHuman organs are still many years away from reality, but scientists have made significant progress in making smaller tissues for specific purposes.
For example, researchers from Tsinghua University also conducted3D bioprintingThe culture of brain-like tissue can cultivate nerve cells. After injecting their additive structures into the cortex of experimental rats, the research team discovered that they can form stimulus response circuits.
on the other hand,3D bioprintingThe company T&R Biofab collaborated with the pharmaceutical company HK inno.N to manufacture a series of artificial skin “test subjects.” The cell structure is part of the disease drugs in an in-depth research program for different skin effects.

Elsewhere, a collaborative group of American universities has adopted another method and developed a method for directly performing tissue treatment in the body.3D bioprintingMethods. The team’s process is based on a novel bio-ink that can construct cells in vitro, thereby eliminating the risk of any potential surgical complications.

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