China3D printingOn March 22, researchers at Lund University in Sweden developed a newCan3D printingBio-ink that can make human organs3D printingOne step closer to reality.
Bio-ink is made from a combination of algae and lung tissue-derived alginate to make biocompatible constructs like human-sized airways3D printing. After printing, the constructs will support the growth of new cells and blood vessels in the graft material.
Although lung tissue was the focus of the initial study, the bioink can reportedly be adapted to any tissue or organ type.
Therefore, the researchers, led by associate professor and senior author of the study Darcy Wagner, believe their work is3D printingFunctional human tissue transplantation provides a promising new bioink.
biology3D printinghuman tissue
Wagner (Transliteration: Wagner) and her team first combined alginate from seaweeds with the extracellular matrix of lung tissue to form a bioink.The bioinks were then loaded with stem cells found in the human airways, and3D printingto form complex and mechanically stable tissue constructs that mimic these airways.
“We started small by making tubules, because that’s a feature found in both the airways and the blood vessels of the lungs,” Wagner said. “By using our new bioink with stem cells isolated from a patient’s airway, we were able to make Bioprinting of small airways that are multi-layered cells and remain open over time.”
3D printingThe constructs include infusible tubes and branch structures that span the anatomical length scales of human tissue and do not require external support structures. The presence of extracellular matrix in the bioink helps to enhance the survival of human progenitor cells (descendants of stem cells that further differentiate into specific cell types), enabling tissue-specific cell differentiation and vascularization in implants during the bioprinting process Formation. transplant site.
For the transplant site, the team used a mouse model that closely resembles the immunosuppressive effects of organ transplant patients. Immunosuppression is a state in which your immune system is not functioning properly, which can be caused by medical procedures such as these.
According to Wagner, the bioink formed from the developed3D printingThe construct inhibits foreign body response, is pro-angiogenic and supports vascularization. This is a result of the bioink’s ability to maintain its biological activity during and after the printing process.
“These next-generation bioinks also support the maturation of airway stem cells into the multiple cell types found in the adult airways, meaning fewer cell types need to be printed, simplifying the process required to print tissues composed of multiple cell types. Number of nozzles,” she explained.
Biocompatibility and angiogenic potential of rECM hydrogels. Image via Lund University.
high resolution creatures3D printing
In order for Wagner and her team to move forward and improve their newly developed bioink, further improvements are needed.3D bioprintingresolution.Higher resolution printing will allow researchers to3D printingMore distal lung tissue and alveoli, which are essential for gas exchange and allow complete3D printinglungs are closer to reality.
She said: “We hope that the available3D printingFurther improvements in machine technology, as well as further development of bioinks, will enable higher resolution bioprinting to engineer larger tissues that can be used for transplantation in the future, and we still have a long way to go. “
Martina De Santis, first author of the study, added: “The development of this novel bioink is an important step forward, but it is important to further validate the function of small airways over time and explore this Feasibility of the method in large aircraft. Animal models.”
Human-derived rECM hydrogels as bioinks for the respiratory tract. Image via Lund University.
biology3D printingProgress
Although cell-filled bioprinted structures hold great potential in human tissue and organ transplantation, the technology is still in the experimental stage. The development of suitable bioinks, limited printing speed, print resolution, and constraints on architectural complexity are all obstacles to the widespread adoption of this technology.
However, over the past year, there have been several important developments that show promise to advance bioprinting and its applications.Scientists at the University at Buffalo have recently developed a fast new3D bioprintingmethod that could bring fully printed human organs closer to reality.The method reportedly enables centimeter-sized hydrogels to be printed faster than conventional3D printingThe technology is 10 to 50 times faster and can also produce embedded vascular networks.
At the same time, the United States3D printingMachine OEM 3D Systems has announced plans to expand its regenerative medicine and bioprinting activities following a breakthrough in its “print-to-perfusion” bioprinting platform. The system is now capable of rapidly producing full-scale vascularized lung stents and will play a key role in the company’s healthcare business.
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