Scientists from the Massachusetts Institute of Technology and the Indian Institute of Technology Madras in a tiny3D printingA small amount of self-organizing brain tissues, so-called organisms, are planted in the system, which can be observed as they grow and develop. This work was reported by AIP Publishing Company in “Biological Microfluidics”.
The current technology for real-time observation of growing organs involves the use of commercial petri dishes with many holes in the glass bottom plate and placed under a microscope. These boards are expensive and only compatible with certain microscopes. They are not allowed to flow or supplement the growing tissues of the nutrient medium.
Recent developments have used a technology called microfluidics, in which nutrient media is delivered through small tubes connected to tiny platforms or chips. However, these microfluidic devices are expensive and difficult to manufacture.The current progress is to use3D printingThe technology creates a reusable and easy-to-adjust platform, and its manufacturing cost is only about $5. The design includes imaging wells and microfluidic channels for growing organisms to provide nutrient media and preheating to support tissue growth.
A biocompatible resin used in dental surgery is used in3D printingDevice. The printed chip is exposed to ultraviolet light for curing, and then sterilized before placing the living cells in the well. After sealing the top of the well with a glass slide, the nutrient media and drugs used in the study were added through a small liquid inlet. The cost of this design is significantly lower than traditional petri dishes or organism culture products based on rotating bioreactors. In addition, the chip can be washed with distilled water, dried and autoclaved, so it can be reused. “
The researchers tested their device with organisms derived from human cells. They observed the growing brain organs with a microscope and successfully tracked their growth and development for 7 days. A small piece of brain tissue develops a cavity or ventricle, surrounded by a self-organizing structure, similar to a developing neocortex.During this week, in3D printingIn the device, the proportion of cell death in the core of the organ is smaller than under normal culture conditions. Researchers believe that their cell design protects the growing tiny brain.
One advantage provided by this microfluidic device is that it allows continuous perfusion of the culture chamber, which is closer to the perfusion of physiological tissue than conventional culture, thereby reducing organoid core cell death. The researchers hope to increase the capacity of their devices by expanding the number of available wells. Other improvements will allow more instruments to be integrated into the design.
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