The 3D printed scaffold vaccine used by Professor Wang Chao from Soochow University’s team “AM” for tumor immunotherapy

As an effective means of tumor immunotherapy, tumor vaccines have received extensive attention from researchers.Here, the team of Professor Wang Chao of Soochow University has developed an immunomodulator3D printingStents are used to enhance tumor immunotherapy.based on3D printingThe rapid manufacturing and precise molding of the cancer vaccine can realize the mass production and personalized design of cancer vaccines. At the same time, compared with traditional gels, the porous structure of the 3D scaffold constructed through modeling and design can allow a large number of immune cells to flow in, giving it a function similar to lymphatic organs, thereby forming an “artificial tertiary lymphatic structure in the body” (aTLS)”. By combining with immune checkpoint therapy, this stent vaccine can better inhibit tumor growth and achieve effective anti-tumor immunity.In addition, combine
clinical
Actually, through
Operation
The construction of a customized tumor resection stent vaccine after resection can also achieve the prevention of tumor metastasis.Therefore, this3D printingStent vaccines are expected to become a potential strategy for cancer vaccine treatment in the future.

First, the team studied
biology
The printing performance of the printing ink. After sodium alginate is loaded with tumor-related antigens, the relevant performance of the bioprinting material has improved, and the printing performance has not changed significantly, which confirms the printing performance of the printing ink and3D printingRapid manufacturing and stable manufacturing. After the tumor scaffold vaccine is manufactured, the morphology of the scaffold vaccine is characterized by scanning electron microscopy, and it can be found that the 3D scaffold vaccine has a good hole structure, which provides good conditions for the infiltration of immune cells. In addition, the 3D scaffold vaccine has good biological safety and will not cause obvious side effects at the implantation site (Figure 1).

Subsequently, the 3D scaffold vaccine can induce the maturation of bone marrow-derived dendritic cells (BMDC) in vitro. Through experiments, it can be found that sodium alginate itself has a certain immunomodulatory effect. The scaffold vaccine loaded with tumor vaccine can more effectively promote the maturation of DC cells, effectively promote the recognition and uptake of antigens by DC cells, and achieve effective immune activation. In addition, the related cytokines secreted by DC cells such as TNF-α, IL-1β, IL-6 and IL-12p70 are also effectively upregulated, which also confirms that the 3D scaffold vaccine can effectively activate DC cells (Figure 2).

By further comparing the immune cell recruitment effects of the 3D scaffold vaccine and the blank scaffold, it was confirmed that the 3D scaffold vaccine can recruit more immune cells, especially T cells, DC cells and macrophages. At the same time, the DC maturity and The level of antigen presentation is effectively up-regulated. In addition, macrophages are also polarized to M1 type, and the level of antigen presentation of macrophages is also significantly up-regulated. Through the recognition and presentation of antigens, the anti-tumor immune response is activated (Figure 3).

(Editor in charge: admin)